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Stem Cell Laboratory at The London Clinic

We offer a range of stem cell processing and cryopreservation services. Our consultant haematologists are available to offer clinical advice on results.

ACCREDITATION AND REGULATION

We are accredited by UKAS and regulated by HTA.

LABORATORY SERVICES

The stem cell laboratory (SCL) carries out the analysis, processing and storage of cells collected from bone marrow and peripheral blood stem cells intended for autologous or allogeneic transplant.

The list of processes performed includes:

  • Peripheral blood stem cell (PBSC) harvest, processing and storage (HPC-A)
  • Bone marrow harvest (BMH), processing and storage (HPC-M)
  • Preparation of T cell therapeutic (TC-T) aliquots, formerly called donor lymphocyte infusions (DLI)
  • Flow cytometry - immunophenotyping for CD34 and lymphocyte subsets CD3, CD4, CD8 and CD19
  • Manipulations that can be arranged include:
  1. Reduction of product volume
  2. Depletion of red blood cells
  3. Concentration of mononuclear cells

Products are cryopreserved in vacuum-packed double bags with 8-10% DMSO cryoprotectant using passive freeze method in a -80°C freezer. Reference sample from cryopreserved cells are frozen and stored in cryovials for further testing if required.

Products are stored exclusively in vapour phase liquid nitrogen vessels, and are monitored (24/7) to ensure maintenance of temperatures between the range of -140 to -190°C

TURNAROUND TIMES

The median turnaround time for CD34 and CD3 analysis by flow cytometry of PBSC, BMHs and DLIs is approximately 90 mins from the time the sample arrives in the laboratory.

COMPLETION OF BOOKING AND REQUEST FORMS

It is important that you provide us with a completed forms by the requesting consultant or transplant coordinator for harvesting and processing including cryopreservation.

The request form should contain the following:

Booking form Request form
  • Patient’s forename and surname
  • Date of birth
  • Hospital L number (if known)
  • Blood Group
  • Weight (kg) of recipient / donor
  • Target dose
  • Type of processing
  • Registry number (VUD only)
  • Donor and recipient consent
  • Patient’s forename and surname
  • Date of birth
  • Hospital L number (if known)
  • Weight (kg) of recipient
  • Product volume
  • Anticoagulant volume
  • Plasma volume
  • Plasma anticoagulant volume

MANDATORY TESTING

It is a regulatory requirement that all patient or donor material processed has undergone HIV 1 & 2, HBV, HCV, HTLV1 and 2, and syphilis testing within the 30 days prior to collection. This testing is carried out by our in-house microbiology laboratory team.

TRANSPORTATION OF PRODUCTS TO THE STEM CELL LABORATORY

Products must be transported within blood transport boxes with cold packs (not freezer packs).  A tracking form and inner container document must also be included.  The stem cell laboratory team will advise on the procedures.

REINFUSION OF CELLS

Requests for issue of fresh and/ or cryopreserved cells for either autologous or allogeneic infusion must be in writing and the recipient consent section must be signed.

Requests must stipulate the date and time of infusion and which bags of stored products are to be infused. 

STORAGE AND DISPOSAL

An audit of cryopreserved material is conducted annually. When materials are nearing the end of the agreed storage time, the requesting clinician will be asked to review if cells are still required. 

Stem Cell Laboratory Key Staff

The London Clinic Staff

Stem Cell Laboratory Manager
(Vacant)

Shoba Satkuru
Stem Cell Laboratory Deputy Manager
E: s.satkuru@thelondonclinic.co.uk

Laboratory Contact Details
T: 0203 219 3326
F: 0203 219 3281

Consultants

Dr Richard Kaczmarski
Medical Director for Processing
Responsible for overseeing the stem cell laboratory and associated activities.

Special interests: Haematological malignancies, blood transfusion medicine, and the investigation and management of anaemias, red cell disorders, coagulation and thrombotic problems.

Appointment enquiries: +44 (0) 20 3944 1073 Concierge service: +44 (0) 203 219 3323 Self-Pay service: +44 (0) 203 918 7301International office: +44 (0) 203 219 3266 Invoice and payment enquiries: +44 (0) 207 616 7708 General enquiries: +44 (0) 20 3918 9135

Breast services appointments: +44 (0) 207 616 7653 Diagnostics appointments: +44 (0) 207 616 7653 Endoscopy appointments: +44 (0) 207 616 7760 Eye centre appointments: +44 (0) 207 616 7768 Haematology appointments: +44 (0) 207 535 5503 Kidney services appointments: +44 (0) 207 224 5234 Liver services appointments: +44 (0) 207 616 7719 Physiotherapy appointments: +44 (0) 207 616 7651 Radiology appointments: +44 (0) 207 616 7653

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